Characterization of chromosomal damage accumulated in freeze-dried mouse spermatozoa preserved under ambient and heat stress conditions
نویسندگان
چکیده
منابع مشابه
Transportation of freeze-dried mouse spermatozoa under different preservation conditions.
Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of ...
متن کاملMaintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa.
Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris.HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was not destroyed by freeze-drying or freezing without cryoprotection in this solution. Embryos derived after injecting oocytes with sperm heads from rehy...
متن کاملMouse and human spermatozoa can be freeze-dried without damaging their chromosomes.
BACKGROUND Although mouse spermatozoa can be freeze-dried without losing their reproductive capacity, the technique needs further improvements to reduce the incidence of chromosomal damage to spermatozoa. Effects of freeze-drying on human spermatozoa are unknown. METHODS Mouse spermatozoa were suspended in a Tris-buffered EGTA solution briefly (10 min at 37 degrees C) or for 1-7 days at 4 deg...
متن کاملChallenging endeavour for preservation of freeze-dried mammalian spermatozoa.
Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose the...
متن کاملEstablishment of Blastocyst Production System with Freeze-dried Bull Spermatozoa
25 Introduction 25 Materials and methods 27 Results 32 Discussion 35 Chapter 4: Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center 39 Abstract 39 Introduction 39 Materials and methods 40 Results 43 Discussion 47 Chapter 5: Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa 49 Abstract 49 Introdu...
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ژورنال
عنوان ژورنال: Mutagenesis
سال: 2011
ISSN: 0267-8357,1464-3804
DOI: 10.1093/mutage/ger003